The biuret test is a chemical test for proteins and polypeptides. These methods are commonly used for determination proteins. The aim of this paper is to show a method to quantify the total protein in tilapia. Biuret synonyms, biuret pronunciation, biuret translation, english dictionary definition of biuret. You may do so in any reasonable manner, but not in. Lowry and bradford methods for measuring hen albumenand egg yolk as protein samples. It is fairly accurate and samples that are out of range can be retested within minutes.
Las proteinas pueden ser estudiadas por diversos y muy diferentes metodos. The test and reagent do not actually contain biuret. Information from its description page there is shown below. The absorbance was measured at a wavelength of 750nm for lowry, 540 nm for biuret and 595 nm for. Learn vocabulary, terms, and more with flashcards, games, and other study tools. It is based on the biuret reagent, a blue solution that turns violet upon contact with proteins, or any substance with peptide bonds. Biuret definition of biuret by the free dictionary. Biuret is a chemical compound with the chemical formula h 2 nco 2 nh. The term biuret also describes a family of organic compounds with the functional grouphnco 2 n. A white, crystalline, nitrogenous substance, c2o2n3h5, formed by heating urea. For last method, bradford, is used as a reagent coomassie brilliant blue g250. The absorbance was measured at a wavelength of 750nm for lowry, 540 nm for biuret and 595 nm for bradford assay. This file is licensed under the creative commons attributionshare alike 3.
12 195 651 884 322 840 547 441 1203 466 1340 499 454 181 840 1202 1432 406 600 510 424 1489 881 312 95 717 1064 388 827 1243 979 1075 308 747 736